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. 2015 Jul 8;19(10):2469–2480. doi: 10.1111/jcmm.12641

Figure 3.

Figure 3

Cx43 regulates aminoglycoside-induced activation of P38. (A and B) Effects of antioxidants and GJ inhibitors on G418-induced activation of P38. Cells were incubated with 5 mM GSH, 10 mM NAC, 7.5 μM α-GA and 10 μM CA for 1 hr before exposing to 600 μg/ml G418 for an additional of 24 hrs. Cellular lysates were subjected to Western blot analysis for total P38 and phosphorylated P38. The percentage of phosphorylated P38 in G418-treated cells with or without GJ inhibitors is shown in the lower panel of B. Quantitative measurement of the band density was performed with ImageJ 1.46 software. The phosphorylated levels of P38 were normalized to total p38, and are expressed as percentage of phosphorylation relative to G418-treated control. Note the obviously reduced level of phosphorylated P38 in antioxidant- or GJ inhibitor-treated cells. (C) Induction of P38 and Cx43 by G418 in foetal fibroblast cells. Cx43+/+, Cx43+/− and Cx43−/− foetal fibroblast cells were treated with or without 600 μg/ml G418 for 12 hrs. Then cellular lysates were subjected to Western blot analysis for P38 and Cx43. Note the different abundance of Cx43 and shift of Cx43 to phosphorylated form in Cx43+/+ and Cx43+/− cells.