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. 2015 Jun 4;80(4):687–697. doi: 10.1111/bcp.12652

Figure 1.

Figure 1

Capture antibodies screened for IL-6 capture in combination with four different detection antibodies. Each candidate antibody was used as a capture antibody to assess 125 pg ml–1 human recombinant IL-6. Detection antibodies from commercially available ELISA kits were used to determine which antibody pair would result in the best detection of IL-6 in human serum. The approach was repeated for human plasma samples, with similar results (data not shown). IL-6, interleukin 6; ELISA, enzyme-linked immunosorbent assay; OD, optical density; mAb, monoclonal antibody; det Ab, detection antibody; Inline graphic Det Ab-1, Inline graphic Det Ab-2, Inline graphic Det Ab-3, Inline graphic Det Ab-4