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Comparison of capture antibodies in the second round of screening. Capture antibodies that passed the broad first round screening were further evaluated in standard ELISA format. Thermo-Pierce 5IL6 (mAb-12; circled) consistently emerged as a superior capture antibody and was included in all ELISAs to normalize ELISA plate runs. ELISA, enzyme-linked immunosorbent assay; OD, optical density; mAb, monoclonal antibody
