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. 2015 Jun 4;80(4):687–697. doi: 10.1111/bcp.12652

Figure 3.

Figure 3

Comparison of top-performing detection and capture antibodies. (A) Four detection antibodies from commercial IL-6 kits were screened and compared for their ability to recognize human recombinant IL-6. Thermo-Pierce 5IL6 was used as a capture antibody. Invitrogen detection antibody (505E23C7) was selected as the detection antibody for future screenings. (B) The three top-performing capture monoclonal anti-IL-6 antibodies (Thermo-Pierce 5IL6, Ebiosciences 16YOR5/66 and Epitomics EBI-R14-19) were compared in a single analysis. The detection antibody was Invitrogen ELISA detection antibody 505E23C7. IL-6, interleukin 6; hIL6, human interleukin 6; ELISA, enzyme-linked immunosorbent assay; OD, optical density; det Ab, detection antibody, Inline graphic R&D det mAb, Inline graphic Thermo-Pierce det mAb, Inline graphic PeproTech det mAb, Inline graphic Invitrogen det mAb, Inline graphic Thermo-Pierce 5IL6, Inline graphic Ebiosciences 16YOR5/66, Inline graphic Epitomics EBI-R14-19