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. 2015 Jun 4;80(4):687–697. doi: 10.1111/bcp.12652

Figure 4.

Figure 4

Final antibody pair selection for determining the best pair that gives the maximum spread of values. Capture/detection antibody pairs evaluated shown respectively: (A) CNTO328/Invitrogen 505E23C7, (B) Thermo-Pierce 5IL6/Invitrogen 505E23C7, (C) Epitomics EBI-R14-19/Invitrogen 505E23C7, (D) MSD IL-6 kit assay and (E) MSD IL-6 kit assay with the Humanzyme IL-6 standard. Solid line indicates LLOQ at 9.77 pg ml–1 and dotted line indicates LLOQ at 1.22 pg ml–1. IL-6, interleukin 6; HS, human serum; RA, rheumatoid arthritis; MSD, Meso Scale Discovery; LLOQ, lower limit of quantification