Pros |
The test has high positive predictive value.
Positive results are highly suggestive of type I immune-mediated reaction.
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The test has been used for a long time and has accumulated a reasonable amount of clinical experience.
Positive result demonstrates the involvement of the immune system in the reaction pathophysiology.
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A reasonable experience with clinical use is available.
In principle the test can be used to predict potential risk to develop a reaction prior to exposure as it detects phenotypic predisposition.
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Recent adaptations of flow cytometric methods have increased its sensitivity.
New techniques allow the use of whole blood samples.
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The test procedure is simplified and more reproducible.
Smaller volume of blood samples is needed.
Has been shown to have a higher sensitivity compared with the LTA.
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Cones |
Low sensitivity and negative predictive value.
Traditionally the technique involves the use of radioactive reagents.
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Expensive and requires highly skilled personnel and sophisticated equipment.
Predictive value is not well defined.
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Procedure is time consuming and demands special skills, resources and reagents.
Has very limited use in few centres.
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Can detect limited types of reactions, which involve basophil activation.
Has low sensitivity.
Available only for a limited number of drugs.
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The test has not been validated by independent group.
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