Fig. 1.

NMDA-R is expressed by cultured Schwann cells. (A) NR1 protein was determined by immunoblot analysis in extracts of rat DRGs, Schwann cells and PC12 cells. Equal amounts of cellular protein (60 μg) were loaded into each lane. Blots were re-probed for β-actin as a loading control. (B) NR1 mRNA was determined by qRT-PCR in Schwann cells transfected with NTC or NR1-specific siRNA (siNR1). (C) Primary cultures of Schwann cells were transfected with NTC siRNA or siNR1. The levels of NR1 and NR2b were determined by immunoblot analysis 48 h after transfection. Blots were re-probed for β-actin as a loading control. (D) Immunofluorescence microscopy was performed to detect NR1 immunoreactivity in Schwann cells transfected with NTC or NR1-specific siRNA. Nuclei were stained with DAPI (blue). Scale bars: 20 μm.