Fig. 5.

tPA initiates NMDA-R-dependent cell signaling in the absence of LRP1 with delayed kinetics. (A) Primary cultures of Schwann cells were pre-treated with 150 nM GST–RAP (+) or with vehicle (−) for 30 min and then EI-tPA (12 nm) for the indicated times. (B) Schwann cells were pretreated with GST–RAP (150 nm) or with vehicle for 30 min and then with increasing concentrations of EI-tPA (12–60 nM) for 10 min. (C) Schwann cells were pre-treated with 150 nM GST–RAP (+) or with vehicle (−) and then α₂M* (10 nM) for up to 60 min. (D) Schwann cells were treated with increasing concentrations of α₂M* for 10 min. Equal amounts of cellular protein (40 μg) were loaded into each lane and immunoblot analysis was performed to detect phosphorylated ERK1/2 (p-ERK) and total ERK1/2 (T-ERK).