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. 2015 Sep 15;128(18):3478–3488. doi: 10.1242/jcs.173765

Fig. 7.

Fig. 7.

Enzymatically active tPA activates ERK1/2 in Schwann cells in a similar manner to EI-tPA. (A) Primary cultures of Schwann cells were pre-treated with 150 nM GST–RAP (+) or with vehicle (−) for 30 min and then with increasing concentrations of enzymatically active tPA (EA-tPA, 12-60 nM) or with 12 nM EI-tPA as a control. (B) Schwann cells were pretreated with GST–RAP (150 nM) or with vehicle for 30 min and then EA-tPA (12 nM) for 15 or 30 min. Cells were treated with 12 nM EI-tPA for 15 min as a control. (C) Schwann cells were pre-treated with 1 µM MK801 (+) or with vehicle (−) for 30 min and then with EA-tPA (12 nM) for 10 min. Equal amounts of cellular protein (40 μg) were loaded into each lane and immunoblot analysis was performed to detect phosphorylated ERK1/2 (p-ERK) and total ERK1/2 (T-ERK).