Table 5.

The primers used for detecting virulence factors of H. pylori

Genes	Primer sequences (5′ → 3′)	PCR product (bp)	PCR conditions
cagA	ACC CTA GTC GGT AAT GGG	521	94 °C, 1 min; 52 °C, 1 min; 72 °C, 1 min (35 cycles)
cagA	GCT TTA GCT TCT GAY ACY GC^a	521	94 °C, 1 min; 52 °C, 1 min; 72 °C, 1 min (35 cycles)
vacA			94 °C, 1 min; 52 °C, 1 min; 72 °C, 1 min (35 cycles)
s1/s2	ATG GAA ATA CAA CAA ACA CAC	259/268
s1/s2	CTG CTT GAA TGC GCC AAA C	259/268
m1/m2	CAA TCT GTC CAA TCA AGC GAG	567/642
m1/m2	GCG TCA AAA TAA TTC CAA GG	567/642
oipA	CCA TGA AAA AAG CTC TCT TAC T	430	94 °C, 30 s; 50 °C, 30 s; 72 °C, 30 s (25 cycles)
oipA	GCC CTT TTA CCC TTC GTT CAA C	430	94 °C, 30 s; 50 °C, 30 s; 72 °C, 30 s (25 cycles)
iceA			94 °C, 1 min; 56 °C, 1 min; 72 °C, 1 min (30 cycles)
iceA1	GTG TTT TTA ACC AAA GTA TC	247
iceA1	CTA TAG CCA STY TCT TTG CA	247
iceA2	GTT GGG TAT ATC ACA ATT TAT	229
iceA2	TTT CCC TAT TTT CTA GTA GGT	229
dupA F2R2	ATG TTT CTT GGT TTA GAG GG	2499	95 °C, 30 s; 56 °C, 30 s; 72 °C, 30 s (35 cycles)
dupA F2R2	TTA TAC ATA TTG AAT ATT CTC GC	2499
dupA F5R3	GGT TTC TAC TGA CAG AGC GC	468
dupA F5R3	CGT ATT TAG TCA GTA AGT TGG CG	468
jhp0562/β-(1,3)galT	TGA AAA GCC CTT TTG ATT TTG	301/602	95 °C, 30 s; 56 °C, 30 s; 72 °C, 30 s (35 cycles)
jhp0562/β-(1,3)galT	GCT GTA GTG GCC ACA TAC ACG	301/602	95 °C, 30 s; 56 °C, 30 s; 72 °C, 30 s (35 cycles)

^aY = C + T