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. Author manuscript; available in PMC: 2015 Oct 6.
Published in final edited form as: Nat Med. 2014 Dec 1;21(1):62–70. doi: 10.1038/nm.3750

Fig. 1. Human HMPS polyps.

Fig. 1

(a) H&E of HMPS polyp showing mixed adenomatous, serrated and dilated cyst morphology and close up of ectopic crypts growing orthogonally to crypt axis. (b) Dysplastic cells (black arrowhead) emerging from an ectopic crypt rather than from the crypt base. (c) Immunostaining of HMPS polyps showing patchy loss of p-SMAD1,5,8 stain, Ki67 stain in proliferating ectopic crypt foci cells and ectopic lysozyme stain in dysplastic crypts (upper panels). Sox9 and EPHB2 immunostaining is increased whereas staining for the differentiation marker CK20, is lost in the ectopic crypt foci of HMPS polyps (lower panels) (n > 10 polyps for all stains). (d) Candidate gene (epi)genetic mutation spectra in HMPS polyps. (e) Laser-capture isolation of individual crypts across HMPS lesions. Spatial distinction of mutant clones allowed inference of mutation timing (see also Supplementary Fig. 2). Scale bars are 100 μm unless stated.

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