Figure 1. Atgl^−/− mice accumulate LDs in neutrophils of peripheral blood and bone marrow.

(A) Relative counts of peripheral blood from WT and Atgl^−/− mice were determined by immunophenotyping by use of flow cytometry. (B) LDs in peripheral blood cells were quantified by use of BODIPY 493/503 as a neutral lipid stain. WT and Atgl^−/− bone marrow were analyzed by transmission electron microscopy (C) and flow cytometry (D and E). LSK, hematopoietic precursors. Data are shown as geometric means of fluorescence intensity + sem (n = 5). ***P ≤ 0.001.