Figure 4. Inflammatory cells express metabolic lipases.

(A) Total RNA of flow-sorted inflammatory cells was isolated and analyzed for mRNA expression of ATGL, HSL, and MGL by quantitative real-time PCR. For data normalization, GAPDH was used as reference gene. Transcript expression levels are shown as fold change, relative to ATGL mRNA levels in macrophages, and represent means + sem (n = 4). (B) Protein levels of lipases in isolated neutrophils were analyzed by Western blotting. Total protein lysate (50 μg) from isolated neutrophils/lane was separated by 10% SDS-PAGE under reducing conditions, blotted onto nitrocellulose membrane, and probed with specific antibodies against ATGL, HSL, and MGL. β-Actin was used as a loading control.