Figure 5. Ca²⁺ flux and chemotaxis are increased in Atgl^−/− neutrophils.

Activity of neutrophils from WT and Atgl^−/− mice was tested in vitro by Ca²⁺ flux (A) and chemotaxis assays (B) in response to fMLP and KC. Data are shown as fold change relative to vehicle-treated control cells ± sem (n = 5). (C) Uptake of BODIPY-labeled FAs by WT and Atgl^−/− neutrophils. Total fluorescence intensities were measured by flow cytometry and are expressed as means + sem (n = 6). (D) Phagocytosis of fluorescein-conjugated E. coli particles by WT and Atgl^−/− neutrophils. Phagocytosis of WT cells was arbitrarily set to 100%. Data are presented as mean values (n = 9–10) + sem. (E) ROS staining after incubation of neutrophils for 20 min with KC (20 ng/ml), fMLP (1 µM), and during 1 h exposure to E. coli particles. Data are shown as total fluorescence intensity (n = 5). *P < 0.05; **P ≤ 0.01; ***P ≤ 0.001.