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Neutrophils were cultured for 6 h in medium in the absence or presence of Atglistatin. Media were collected from thioglycolate-elicited neutrophils and activated neutrophils (0.5 µM A23187, 1 h). PUFA and lipid mediator concentrations were measured by LC-MS/MS analysis and are presented as median and range (n = 6). Ai, Atglistatin; n.d., not detectable; Thio, thioglycolate. *P < 0.05.
