Figure 4. MET is required for neutrophil transendothelial migration and cytotoxicity.

a-c, Neutrophils quantification (a) on TPA-painted ear skin (b,c). Data combine 2 independent experiments; total mice=10/condition for vehicle, 14/condition for TPA. d, FACS analysis for Ly6G⁺ neutrophils or F4/80⁺ macrophages on peritoneal lavages after zymosan-induced peritonitis. Data are representative of 2 independent experiments using 4 mice/condition per experiment. e, FACS analysis for neutrophil recruitment towards HGF or CXCL1 in air pouch assays. Data combine 3 independent experiments; total mice=10/condition. f, Ly6G infiltration in LLC tumours or in TPA-painted ear skin after anti-HGF. Data combine 2 independent experiments; total mice: IgG=11, anti-HGF=6. g-i, Neutrophil adhesion to HUVEC/IL (g) and transendothelial migration in response to HGF (h) or TCM with or without anti-HGF (i). Data in (g-i) are representative of 3 independent experiments using 3 biological replicates/condition per experiment. j,k, qRT-PCR for Nos2 in LLC-tumour-associated neutrophils or macrophages (j) and tumour-derived NO production (k). l-n, Quantification (l) and representative images (m,n) of 3NT and Ly6G-costained LLC tumour sections. Data in (j-n) combine 2 independent experiments; total mice=8/condition. o, TAN cytotoxicity against LLC cells with or without L-NMMA. Data are representative of 4 independent experiments using 3 biological replicates/condition per experiment. p-r, LLC tumour growth (p), weight (q), TANs (r) following neutrophil-depleting anti-Ly6G treatment. Data combine 2 independent experiments; total mice=16/condition. *, P<0.05; #, P<0.05 versus PBS (e), versus Mock (g-i), versus WT→WT untreated (o). Scale bars: 100 μm (b,c), 20 μm (m,n). Graphs show mean ± s.e.m..