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. Author manuscript; available in PMC: 2015 Oct 6.
Published in final edited form as: Dev Dyn. 2010 Jan;239(1):284–296. doi: 10.1002/dvdy.22137

Figure 3. The hippocampus develops normally in Wnt8b−/− mutants.

Figure 3

(A-C’) In situ hybridisation for Lef1 in E12.5 wild type (A,B,C) and Wnt8b−/− mice (A’,B’,C’). The areas boxed in B and B’ are magnified in C and C’, respectively. The expression pattern of Lef1 is indistinguishable between wild types and mutants. Arrowheads in C and C’ indicate lack of Lef1 expression in the cortical hem (Hem) and choroid plexus (ChP). (D) Bar graph showing BrdU labelling indices in the dorsomedial telencephalon of Wnt8b−/− mutants and wild type controls at E14.5. n=3 animals of each genotype, error bars indicate SEM. (E-G’) In situ hybridisation on coronal sections of E18.5 wild type (E,F,G) and Wnt8b−/− (E’,F’,G’). NP2 marks the entire hippocampus (E,E’), while Prox1 (F,F’) and SCIP (G,G’) mark the dentate gyrus (DG) and CA1 field of the hippocampus respectively. Scale bars: A-B’, 200 μm; C-C’, 100 μm E-G’, 250 μm. Abbreviations: Hi: hippocampus; nctx: neocortex.