Figure 4.

Trophoblast giant cells (TGC) in WT and Plac1-mutant placentae. In situ hybridization of Prl2c2 expression using the digoxygenin detection system, as described in “Materials and Methods”. Black arrows = parietal TGCs adjacent to the decidua; Green arrow = sinusoidal TGCs; Red arrow = SpT at the interface of the junctional zone and labyrinth. Non-specific signal was determined by hybridization with the “sense” riboprobe construct. Magnification = 1.5×.