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. 2015 Oct 6;15:33. doi: 10.1186/s12861-015-0083-8

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© Wright et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Expression patterns of genes encoding Wnt ligands and receptors are unchanged in Spry1 ^−/− ; Spry2 ^−/− embryos. a – j In situ hybridization analyses of genes encoding Wnt ligands and receptors in Spry1 ^−/− ; Spry2 ^−/− embryos and controls at OEPD or early otic placode stages. Lateral views of whole-mount embryos are shown; embryos are oriented as indicated. Pre-otic regions are bracketed. k Semi-quantitative reverse-transcriptase PCR analyses of candidate Wnt and Fzd transcript levels in RNA collected from 5 – 7 s hindbrain and OEPD-containing tissue microdissected from the genotypes indicated. Wnt genes (Wnt1, Wnt3a, Wnt6, and Wnt8a) with known expression adjacent to the OEPD are controls. Scale bar, 100 μm