Table 3.

Top 25 highly expressed G protein-coupled receptors in HEMs

Gene Name (Gene ID)	Average FPKM ± SEM
G protein-coupled receptor 143 (OA1)	254.7 ± 44.0
endothelin receptor type B (EDNRB)	211.6 ± 43.0
G protein-coupled receptor 56 (GPR56)	148.8 ± 29.3
G protein-coupled receptor 137B (GPR137B)	117.4 ± 7.1
5-hydroxytryptamine (serotonin) receptor 2B (HTR2B)	66.4 ± 37.5
cysteinyl leukotriene receptor 2 (CYSLTR2)	29.5 ± 14.4
G protein-coupled receptor 107 (GPR107)	25.4 ± 2.6
coagulation factor II (thrombin) receptor (F2R)	24.6 ± 9.2
G protein-coupled receptor 108 (GPR108)	21.5 ± 3.6
G protein-coupled receptor 125 (GPR125)	21.2 ± 1.5
G protein-coupled receptor 175 (GPR175)	14.6 ± 3.3
G protein-coupled receptor, fam. C, group 5, member A (GPRC5A)	13.3 ± 1.9
melanocortin 1 receptor (MC1R)	12.8 ± 4.9
cadherin, EGF LAG seven-pass G-type receptor 2 (CELSR2)	11.9 ± 3.0
G protein-coupled receptor 124 (GPR124)	11.3 ± 1.9
CD97 molecule (CD97)	10.9 ± 1.8
encephalopsin (OPN3)	10.1 ± 1.5
G protein-coupled receptor, fam. C, group 5, member B (GPRC5B)	9.5 ± 3.8
smoothened, frizzled family receptor (SMO)	8.8 ± 1.3
lysophosphatidic acid receptor 6 (LPAR6)	8.5 ± 3.9
frizzled family receptor 6 (FZD6)	8.5 ± 3.1
G protein-coupled receptor 137 (GPR137)	8.2 ± 1.3
gonadotropin-releasing hormone (type 2) receptor 2 (GNRHR2)	7.9 ± 0.7
gamma-aminobutyric acid (GABA) B receptor, 1 (GABBR1)	7.9 ± 1.4
G protein-coupled receptor 155 (GPR155)	7.8 ± 1.4

The highest expressed GPCRs in melanocytes were obtained by averaging their FPKM from each library. The mean expression levels of orphan GPCRs shown in dark grey background and receptors known to be important for melanocyte function (GPR143, EDNRB, and MC1R) are in bold.