Fig. 7.

Effects of CR on the expression of autophagy markers and phosphorylated AMPK in islets. a LC3, beclin-1 and p62 protein levels in freshly isolated islets were determined by western blot, β-actin was used as an internal standard and representative blots were shown. b the ratio of LC3-II to LC3-I(n = 6). c and d the protein expressions of beclin-1 and p62 relative to β-actin (n = 6). e Representative blots of phosphorylated and total AMPKα protein expression in freshly isolated islets. f the ratio of pT172-AMPKα to total AMPKα (n = 6). Data were presented as means ± SE, a indicates P < 0.05 versus NC AL group; b indicates P < 0.05 versus HF AL group