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. 2015 Oct 6;11(10):e1005556. doi: 10.1371/journal.pgen.1005556

Fig 4. The amount of plasmid DNA associated with Ssb-GFP was decreased in the plasmid with sso1.

Fig 4

We measured the relative amount of plasmid DNA associated with Ssb-GFP (A) and the relative amount of total plasmid (B), for plasmids with and without sso1. Strains containing the indicated plasmid were: CMJ129 (pHP13); CMJ130 (pHP13sso1); and CMJ131 (pHP13sso1R). (A) Association of plasmid DNA with Ssb-GFP was measured by chromatin immunoprecipitation (ChIP) with polyclonal anti-GFP followed by qPCR with primers to detect cat in pHP13. The plasmid qPCR signal from immunoprecipitated DNA was normalized to the qPCR signal in pre-immunoprecipitation lysates (% of input) [42]. Data are from one representative experiment of 6 independent experiments. Error bars indicate the standard deviation of technical triplicates. (B) Relative quantification of plasmid DNA was determined by qPCR. Plasmid abundance (pHP13 cat) was normalized to a control chromosomal locus (ydbT), and the abundance of pHP13sso1 and pHP13sso1R was normalized relative to pHP13. Data are averages from 5 independent experiments ± standard deviation.