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. 2015 Oct 6;11(10):e1005555. doi: 10.1371/journal.pgen.1005555

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© 2015 Koludrovic et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — A. Western blot showing knockdown of BPTF and MITF in Hermes-3A cells. B. Numbers of Hermes-3A cells measured using a MTT cell proliferation kit following shBPTF knockdown. C. Phase contrast microscopy of Hermes-3A cells following shBPTF and shMITF knockdown. Magnification X20. Inlays show enlargements. D. Staining and quantification of Hermes-3A cells following shBPTF and shMITF knockdown for senescence-associated β-galactosidase. E. Venn diagrams illustrate the overlap between up and down-regulated genes following shBPTF and shMITF knockdown. RF shows the representation factor and p value for the overlaps between the up and down-regulated genes sets. Several examples of commonly regulated up and down-regulated genes are indicated based on the results of RNA-seq. F. Ontology of genes up and down-regulated by shBPTF and shMITF knockdown and of commonly regulated genes in Hermes 3A cells.