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. Author manuscript; available in PMC: 2015 Oct 6.
Published in final edited form as: Immunol Rev. 2013 Nov;256(1):95–106. doi: 10.1111/imr.12116

Fig. 3. Ca2+ signaling is required for optimal T-cell-antigen-presenting cell conjugate formation.

Fig. 3

OT-1 T-cell blasts were stained with CFSE and mixed with antigen-loaded, PKH26-stained RMA-s cells in the presence or absence of Ca2+ chelators as indicated. After incubation at 37°C for the indicated times, samples were analyzed by flow cytometry and conjugates identified as GFP+ PKH26+ entities. Percent conjugates = [GFP+PKH26+/(GFP+PKH26+ GFP+PKH26+)]. Error bars indicate standard error of the mean for triplicate measurements. Data are representative of two independent experiments.