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. Author manuscript; available in PMC: 2016 Jun 25.
Published in final edited form as: Neuroscience. 2015 Mar 27;297:22–37. doi: 10.1016/j.neuroscience.2015.03.050

Fig1. Western blot and immunofluorescent microscopy (see Experimental Procedures) indicate that the D-12 5-HT2CR antibody used throughout this study is specific for the 5-HT2CR.

Fig1

POIC cells that express 5-HT2CRs consistently showed D-12 5-HT2CR-antibody expression under both Western Blot (see A, black bars in histogram) and Immunohistochemical assessment (see B, confocal microscopy image of green cellular 5-HT2CR immunofluorescence). However, GF62 cells that only express 5-HT2AR s showed no D-12 antibody expression in either test (see A and B). Scale bar = 20 µm.