Fig. 2.

Molecular detection of simian malaria parasites in long-tailed macaques by nested PCR. The PCR products were subjected to electrophoresis in 2 % agarose gel. Here, the gel pictures show the positive and negative cases of four different species: P. knowlesi, P. cynomolgi, P. coatneyi, P. inui and P. fieldi. Here, DL indicates 50–100 bp ladder, PC and NC stands for positive and negative control. The expected product sizes for P. knowlesi, P. cynomolgi, P. coatneyi, P. inui and P. fieldi are 153, 137, 504, 479 and 421 bp