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. 2015 Oct 5;14:156. doi: 10.1186/s12934-015-0348-8

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© Rahmen et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Relative changes in intracellular nucleobases during initial growth and production phases. Time-dependent changes in free nucleobases within the cells presented for six clones varying in Arg107 codons (left) and six clones varying in Leu143 codons (right) as relative intensities in [%]. At the indicated time points, the cells were collected, their metabolites extracted, and the nucleobases were analyzed by LC/MS. Clones categorized into Type A respiration behavior are presented with fully filled bars, Type B clones are indicated with striped bars. The three chosen time points represent initial growth on glucose (3 h) and glycerol (4 h), and recombinant protein production on lactose (8 h). All data points are the mean of triplicate measurements. The error bars represent the standard deviation