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. Author manuscript; available in PMC: 2015 Oct 6.
Published in final edited form as: Allergol Int. 2015 Jul 10;64(0):S30–S36. doi: 10.1016/j.alit.2015.04.009

Fig. 3.

Fig. 3

Effects of ATPγS on eosinophil trans-basement membrane migration by engagement of P2 receptors. (A) Eosinophil suspensions with or without 100 µM ATPγS were added to the upper chamber, and 100 µM ATPγS was added to the lower chamber in a checkerboard fashion. Eotaxin (100 ng/ml) in the lower chamber was used as a positive control. After 2 h at 37 °C, the cells that migrated to the lower chambers were collected and counted using light microscopy. The data show the percentage of migrated cells to the total number of initial cells. Results show the mean ± SEM from five different eosinophil preparations. p < 0.01, compared with medium alone in the lower chamber. (B) Eosinophils were pre-incubated with or without 30 mM oATP at 4 °C for 15 min. Subsequently, eosinophil suspensions were added to the upper chamber and 100 µM ATPγS was added to the lower chamber. The migration ratio was determined as described in (A). Results show the mean ± SEM from five different eosinophil preparations. p < 0.01, compared with medium alone in the lower chamber.