Fig. 7.

Effect of exogenous ET-1 on LIMK2 expression and mitochondrial fission at 3 days after injection. a-c Representative photographs of LIMK2 and NeuN in the CA1. As compared to vehicle, ET-1 injection increases LIMK2 expression accompanied by reduction in NeuN expression. Co-treatment of ET-1 and BQ788 attenuates up-regulation of LIMK2 expression induced by ET-1. Bar = 100 μm. d Quantitative values (mean ± S.E.M) of LIMK2 and NeuN (n = 10 per each group). Significant differences from vehicle, *p < 0.05. e Quantitative values (mean ± S.E.M) of mitochondrial length (n = 10 per each group). Significant differences from vehicle, *p < 0.05. f-h Representative photographs of mitochondria and NeuN in the CA1 neurons. ET-1 injection increases mitochondrial length and sphere formation, as compared to vehicle. Co-treatment of ET-1 and BQ788 prevents mitochondrial elongation and sphere formation induced by ET-1. Bar = 6.25 μm