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. 2015 Oct 5;14:154. doi: 10.1186/s12934-015-0344-z

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© Yang et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Cre-mediated excision of the selectable marker. After Cre induction and incubation, potential Δgnp::lox72 mutants were inspected by PCR testing using primers gnp-testA and gnp-testB. Lane 1 shows DNA ladder, lane 2 was the Δgnp::cat strain. Lanes 3–11 were tested colonies, with marker-free mutants expected to generate amplicons of ∼3.2 kb