Fig. 1.

Binding of extracellular matrix proteins by Candida spp. filamentous forms. a Fibronectin (FN) binding by C. albicans, C. parapsilosis and C. tropicalis; b vitronectin (VTN) and laminin (LAM) binding by C. parapsilosis and C. tropicalis. Filamentous forms obtained from 1 × 10⁶ yeast cells and adsorbed in wells of MaxiSorp microplates were incubated at 37 °C for 1.5 h with 50 μl of biotin-labeled FN, VTN or LAM solutions. Additional wells without adsorbed fungal cells but with BSA-blocked surfaces served as controls. The amounts of bound protein were determined using an SA-HRP/TMB system. The readings from control wells were subtracted from the total binding values. Representative binding plots are presented, in which data points represent means from three determinations (three independent wells) ± standard deviation