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. 2015 Oct 5;15:197. doi: 10.1186/s12866-015-0531-4

Table 2.

Candida albicans proteins with the previously confirmed binding affinity to human fibronectin, vitronectin and laminin

Abbreviation Protein name Method used for protein identification and confirmation of binding to ECMPs Reference
Fibronectin-binding proteins
Als1 Agglutinin-like sequence protein 1 expression in Saccharomyces cerevisiae followed by binding to magnetic beads coated with fibronectin [38]
expression in S. cerevisiae followed by binding to microplate-immobilized fibronectin [16]
expression of the Als1 N-terminal part in S. cerevisiae followed by its purification and surface plasmon resonance measurements [39]
Als3 Agglutinin-like sequence protein 3 expression in S. cerevisiae followed by binding to microplate-immobilized fibronectin [16]
expression in S. cerevisiae followed by microtiter plate-based assay [40]
Als5 Agglutinin-like sequence protein 5 expression in S. cerevisiae followed by binding to microplate-immobilized fibronectin [16]
binding of protein fragments—Als5 1–431 and Als5 1-664—to fibronectin-coated microtiter plate wells [41]
Hwp1 Hyphal cell wall protein 1 expression in S. cerevisiae followed by microtiter plate-based assay [40]
Tdh3 Glyceraldedyhde-3-phosphate dehydrogenase immunoaffinity purification of Tdh3 followed by ligand Western Blot assay [18]
Adh1 Alcohol dehydrogenase screening of C. albicans cDNA library with polyclonal antibodies against receptor for fibronectin [17]
Vitronectin-binding proteins
Adh1 Alcohol dehydrogenase screening of C. albicans cDNA library with polyclonal antibodies against receptor for vitronectin [17]
Gpm1 Phosphoglycerate mutase enzyme-linked ligand sorbent assay (ELISA) with microplate-immobilized recombinant Gpm1 [45]
Laminin-binding proteins
Als1 Agglutinin-like sequence protein 1 expression in S. cerevisiae followed by binding to magnetic beads coated with laminin [38]
expression in S. cerevisiae followed by binding to microplate-immobilized laminin [16]
expression of the Als1 N-terminal part in S. cerevisiae followed by its purification and surface plasmon resonance measurements [39]
Als3 Agglutinin-like sequence protein 3 expression in S. cerevisiae followed by binding to microplate-immobilized laminin [16]
Als5 Agglutinin-like sequence protein 5 expression in S. cerevisiae followed by binding to microplate-immobilized laminin [16]
Tdh3 Glyceraldedyhde-3-phosphate dehydrogenase immunoaffinity purification of Tdh3 followed by ligand Western Blot assay [18]