Fig. 5.

Expression profile of the genes in terpenoid backbone pathway in the grape berries. a Pathway of terpene biosynthesis in grape berries; The MEP pathway is localized in plastids, while the MVA pathway occurs in the cytosol. The following enzymes and metabolites are shown: G3P glyceraldehyde 3-phosphate, DXS 1-deoxy-D-xylulose-5-phosphate synthase, DXR 1-deoxy-D-xylulose 5-phosphate reductoisomerase, MEP 2-C-methyl-D-erythritol 4-phosphate, MCT 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, CDP-ME 4-(Cytidine 5'-diphospho)-2-C-methyl-D-erythritol, CMK 4-(cytidine 5’-diphospho)-2-C-methyl-D-erythritol kinase, CDP-MEP 2-Phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol, MDS 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, ME-Cpp 2-C-Methyl-D-erythritol 2,4-cyclodiphosphate, HDS 4-hydroxy-3-methylbut-2-enyldiphosphate (HMBPP) synthase, HMB-PP (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate, HDR 1-hydroxy-2-methyl- 2-(E)-butenyl-4-diphosphate reductase, IPP isopentenyl pyrophosphate, DMAPP dimethylallyl pyrophosphate, IPPI IPP-isomerase, GPPS geranyl pyrophosphate synthase, GPP geranylpyrophosphate; AACT acetoacetyl-CoA thiolase, HMGS 3-hydroxy-3-methylglutaryl synthase, HMG-CoA 3-hydroxy-3-methylglutaryl-CoA, HMGR 3-hydroxy-3-methylglutaryl-CoA reductase, MVA Mevalonate, MK MVA kinase, MVP Mevalonate-5-phosphate, PMK phospho-MVA kinase, MVPP Mevalonate-5-diphosphate, MPDC diphospho-MVA decarboxylase, MVPP mevalonate-5-pyrophosphate, FPPS farnesyl pyrophosphate synthase. b Transcription profile of the genes in the MEP and MVA pathway. Each row represents an individual gene and each column represents an individual sample. The data was normalized by rows used function “scale”. The topographycal colors are installed in deep red and deep blue, which depict relative expression abundances of genes from high to low. The color scale bar is shown at the right of the heat map. Dendrograms indicate the correlation between groups of genes. c Expression of two main genes in the MEP pathway