Fig 3. Characterization of μOrgano building blocks.

a) Transition time of the interface of a liquid advancing through a system of two MPSs and a linear connector. The time necessary to advance from the cell chamber in MPS 1 to the cell chamber in MPS 2 is plotted versus the inner diameters of the glass capillaries in the respective systems. Insets show pictures of the respective glass capillaries (scale bars = 2 mm). b) Scatter plot of the transition times for ten independent systems connected by the same type of connectors featuring 50 μm ID capillaries. c) Time series of microscopy images from a channel section in the proximity of the inlet of the second MPS initially filled with clear water. The continuous transition occurring after connection to a MPS filled with coloured water using a food dye reveals the bubble less connection ability of the system (scale bar = 100 μm). d) Time series of pictures showing two MPSs connected by a linear connector whereby MPS 1 is prefilled with red dyed water, and MPS 2 and the connector with blue dyed water. Pumping red dyed water into MPS 1 leads to the replacement of the blue dyed water in both the connector and MPS 2 without the occurrence of leakage. e) Volume flown through MPS 2 (left; in flow direction) and MPS 3 (right) plotted as percentage of the total volume after connection to MPS 1 via a bifurcation connector.