Fig 4. Proof of concept of the μOrgano system.

a) General procedure for biological experiments with the μOrgano system. b) Combined culture of two devices with 3T3 fibroblasts: Live (green) /dead (red) staining in both devices after 1 day of individual and 2 days of combined culture show that viability can be maintained. c) In-series culture of two heart-on-a-chip devices: tracings of the beating motion of cardiac tissue formed by hiPSC-cardiomyocytes—i) optical microscopy image—in two connected MPSs (ii) MPS 1; iii) MPS 2). The analysis using computational motion tracking reveals that a physiological phenotype is retained and individual cardiac devices beat with distinct frequencies. (scale bars = 200 μm).