Table 1.
Primary antibodies used in the study
| Antibodies | Type | Source | Dilution | Measuring site |
|---|---|---|---|---|
| CD3 | Mouse monoclonal | DakoCytomation® | 1:100 | All T-lymphocyte |
| CD8 | Mouse monoclonal | DakoCytomation® | 1:100 | Cytotoxic T-lymphocyte |
| CD68 | Mouse monoclonal | DakoCytomation® | 1:200 | Macrophage |
| Neutrophil elastase | Mouse monoclonal | DakoCytomation® | 1:200 | Neutrophil |
| IL-33 | Rabbit polyclonal | Milipore® | 1:100 | Bronchial epithelium |
| γ-ENaC | Rabbit polyclonal | Milipore® | 1:100 | Bronchial epithelium Vascular smooth muscle cell |
| AQP-1 | Rabbit polyclonal | Milipore® | 1:100 | Alveolar capillary |
| AQP-5 | Rabbit polyclonal | Milipore® | 1:50 | Alveolar epithelium |
| Pan cytokeratin | Rabbit polyclonal | Milipore® | 1:400 | Bronchial epithelium |