Fig. 3.

γ-PGA promotes NK cells’ cytotoxicity and enhances DCs’ maturation in influenza virus-infected mice. C57BL/6 mice (n = 5) were infected with PR8 and CA04 influenza viruses at 1 LD₅₀ per mouse. After 24 h, mice were intranasally administrated with 100 μg of γ-PGA and 5 μg of CpG-ODN administrated prior to virus infection for 24 h. a Lung cell suspensions were magnetically separated to purify NK cells by positive selection using the CD49b antibody. Purified NK cells were mixed with target cells at an effector cell to target cell ratio of 50:1, and specific lysis values were quantified by performing lactate dehydrogenase release assays. b Cell suspensions were stained with anti-mouse CD86 antibody. To avoid nonspecific counting, cells were gated with anti-mouse CD11c + antibody and then DCs’ maturation was measured by flow cytometry. In each overlay, unfilled histograms represent the expression of maturation marker on lung DCs. Data are representative of two independent experiments