Fig 5. Kamebakaurin causes multiple spindle defects and misaligned chromosomes.

(A) Metaphase spindle defects in NIH 3T3 cells treated with kamebakaurin (50μM). Asterisks indicate diffused pericentrin distribution. Scale bar: 5 μm. (B) Quantification of the percentage of cells with abnormal spindles in kamebakaurin- (50μM) or DMSO (control)-treated groups. More than 100 cells were counted in each group. The data are presented as the mean ± SD. (C, D) Kamebakaurin has no effect on the polymerization of tubulin or actin in in vitro assays. Vinblastine and cytochalasin D are known tubulin and actin inhibitors, respectively.