Fig 7. KIF7 regulates microtubule mstability in mouse lung epithelial (MLE15) cells.

(A.) Growth curves of control (Scram) and KIF7 depleted cells following treatment and washout with the microtubule stabilizing drug, paclitaxel (TAX), and the microtubule depolymerizing drug, nocodazole (NOC). Cells were counted and plated, and then treated 24 hours later (Day 1). 18 hours later (Day 2), cells were washed 2 times in PBS, and then incubated with complete media. (B.-C.) Flow cytometric analysis of DNA content after overnight incubation with paclitaxel. (D.+D’) Confocal Z stacks of immunofluorescent staining of acetylated alpha tubulin in control and KIF7 depleted MLE15 cells. (E.) Western blot analysis of high passage asynchronous MLE15 cells. N≥3, * P<0.05, **P<0.01.