Fig. 4. Repeated passive exercise in mice that had been subjected to chronic repeated stress produced changes in physiological parameters in the blood. (A) Experimental design for treatment with 2 hours×14 days of restraint (RST), and post-stress treatment with exercise (EXE). Mice were treated with 2 hours×14 days RST only, and with 2 hours×14 days RST followed by post-stress treatment with 1 hour of exercise for 14 days (RST+EXE 14d), and 21 days (RST+EXE 21d). Physiological blood parameters were analyzed with i-STAT CG4⁺ cartridge and the i-STAT 1 analyzer from Abbott. (B) Lactate levels in the blood of control (CON) mice, mice treated with 2 hours×14 days RST only, 2 hours×14 days RST+EXE 14d, and 2 hours×14 days RST+EXE 21d. (C) Blood pH of CON mice, mice treated with 2 hours×14 days RST only, 2 hours×14 days RST+EXE 14d, and 2 hours×14 days RST+EXE 21d. (D, E) Partial pressures of O₂ (pO₂, D) and saturated O₂ (sO₂, E) levels in the blood of CON mice, mice treated with 2 hours×14 days RST only, 2 hours×14 days RST+EXE 14d, and 2 hours×14 days RST+EXE 21d. (F-I) Partial pressures of CO₂ (pCO₂, F), total CO₂ (TCO₂, G), HCO₃ (H), and base excess extra cellular fluid (BEecf, I) levels in the blood of CON mice, mice treated with 2 hours×14 days RST only, 2 hours×14 days RST+EXE 14d, and 2 hours×14 days RST+EXE 21d. Values are expressed as mean±SEM (n=5 to 15). Difference between the indicated group at ^aP<0.05; ^bP<0.01, respectively (one-way analysis of variance followed by the Newman-Keuls test).