Table 1. PCR primers used for nested PCR.
| SNP: Genome location | Primers (5’-3’)* | Amplicon size (bp) | Annealing temperature (°C) | |
|---|---|---|---|---|
| 14 676 | Outer | F1: CAGGTCTTGTGCGGATAA | 337 | 51 |
| R1: AGGACACCTTCGACATGG | ||||
| Inner | F2: AATGGAATGCTGGTGAGAGC [6] | 194 | 56 | |
| R2: CAATGCATGCTAGCCTTAATGA [6] | ||||
| 1 642 875 | Outer | F3: TGCTAGTTTAACCGAGTACTGCTA [6] | 287 | 47,5 |
| R3: GTACAACTCATAAGCACG | ||||
| Inner | F4: TAACTGGGCGCAGAGGAA | 162 | 56 | |
| R4: GTAGTAGTCTTCCAAGTTGTGGTG [6] | ||||
| 2 935 685 | Outer | F5: ATCTGGTCCGGGTAGGAATC [6] | 268 | 51 |
| R5: CGTGCTGACTGTCATGAT | ||||
| Inner | F6: TACGGTGGTGTCGGTCTC | 123 | 56 | |
| R6: ACCGGTGAGCGCACTAAG [6] | ||||
Primers’ genome location: F1-14 492; R1-14 828; F2-14 528; R2-14 721; F3-1 642 737; R3-1 643 023; F4-1 642 764; R4-1 642 925; F5-2 935 537; R5-2 935 804; F6-2 935 594; R6-2 935 716