Table 1.

Transcriptome Read Depth for Each Sample Library

Library	Total Reads	Mapped Reads	Unique Reads
Control 1	26,481,906	89.9 %	95.9 %
Control 2	35,224,137	89.8 %	96.4 %
Control 3	35,841,457	90.4 %	95.8 %
Control 4	22,491,917	87.8 %	96.0 %
Control 5	17,147,757	87.3 %	96.5 %
Control 6	13,106,307	87.4 %	96.6 %
Heat stress 1	33,816,428	90.0 %	95.2 %
Heat stress 2	13,710,771	90.0 %	95.8 %
Heat stress 3	27,526,906	90.4 %	96.0 %
Heat stress 4	19,754,286	87.6 %	96.2 %
Heat stress 5	26,463,555	87.7 %	96.1 %
Heat stress 6	31,610,408	88.2 %	96.2 %

The total number of sequences from each library prepared from separate flasks of either control (37 °C) or heat stressed (43 °C) LMH cells is given in the Total Reads column. The Mapped Reads column is the percentage of these total reads that mapped to the chicken genome. The Unique Reads column is the percentage of mapped reads that only correspond to one site in the genome. Combined, this data yielded more than 25 × 10⁷ reads that mapped uniquely to the chicken genome.