Fig. 1.

Resveratrol significantly induces transcription activities of the HSP70 promoter and ARE and enhances MSC survival on CSE challenge. a Schematic representation of the lentiviral vectors. The 2A peptide sequence (T2A) from the Thosea asigna virus was used as a linker connecting the red fluorescence protein (DsRed), hygromycin and the luciferase (Luc) gene to generate the lentiviral multi-cistronic expression vector, pLenti-LT-hyg. The pLenti-8XARE-Luciferase-DsRed-hygromycin (abbreviated as 8XARE-Luc), pLenti-HSPp-Luciferase-DsRed-hygromycin (HSP-Luc), and pLenti-HSP70p-VEGFA-Luciferase-DsRed-hygromycin (HSP-VEGFA). b HSP-Luc-MSC or c ARE-Luc-MSC was co-treated with the indicated concentrations of c-RSV or t-RSV and CSE for 12 h. At the end of the treatment, cells were harvested for luciferase assays. d HSP-VEGFA-MSC was similarly co-treated with c-RSV or t-RSV and CSE for 48 h, followed by cell viability MTT assay. Data represent means ± SD of three independent experiments. *P < 0.05 and **P < 0.01 indicate differences between the treated cells and the mock control