Skip to main content
. 2015 Oct 6;10(10):e0139809. doi: 10.1371/journal.pone.0139809

Fig 3. Gemcitabine-induced upregulation of the expression of NKG2D ligands is regulated via ATM-ATR pathway in A549 cells.

Fig 3

(A): Histograms demonstrating MHC class I molecules and NKG2D ligands in A549 cells treated with 5, 10 mM of Caffeine or 30μM of KU55933 or 5mM of N-acetyl-L-cysteine (NAC) for 24 hours. (B): Histograms demonstrating MHC class I and NKG2D ligands in A549 cells pretreated with Caffeine or KU55933 or NAC for 2 hours followed by Gemcitabine (GEM) at 10 nM for 24 hours. Data are representative of three independent experiments. NT: no treatment control. (Ci) A549 cells were treated with Caffeine or KU55933 or for 24 hours. The phosphorylated-ATM (pATM) was assessed by flow cytometry then the effects on the expressions of pATM treated with Caffeine or KU55933 were shown as the relative MFI (rMFI) mean values of four independent experiments and evaluated with Student t-test. (ii) A549 cells were pretreated with Caffeine or KU55933 for 2 hours followed by Gemcitabine for 24 hours then the pATM was assessed by flow cytometry. The effects on the expressions of pATM were shown as the rMFI mean values of at least three independent experiments and evaluated with Student t-test. (D): MHC class I molecules and NKG2D ligands in A549 cells treated with siRNA of ATM for 24 hours followed by Gemcitabine (GEM) at 10 nM for 24 hours. Representative histograms of three independent experiments are shown. The relative MFI (rMFI) of MHC class I molecules and NKG2D ligands were calculated based on at least three independent experiments and evaluated with a Student t-test. Bars, SEM. * -p<0.05 and ** -p<0.01.