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. 2015 Oct 6;11(10):e1005542. doi: 10.1371/journal.pgen.1005542

Fig 3. EPRAP deficiency impairs the anti-inflammatory effect of PGE/EP4.

Fig 3

(A) Representative photographs of colons; “Veh” indicates vehicle and “EP4” indicates EP4 agonist. (B) Percent changes in colon length expressed relative to DSS-free water controls (n = 5–8 each; **P < 0.01 vs. WT + Veh). (C) Histological colitis scores (n = 5–8 each; *P < 0.05 vs. WT + Veh). (D) Representative H&E staining. Scale bars: 100 μm. (E, F) Peritoneal macrophages (E) and colonic epithelial cells (F) isolated from WT and KO mice were incubated with EP4 agonist. Intracellular cAMP levels were measured as described in Supplemental Methods (*P < 0.05 and **P < 0.01 vs. 0 μM). Figures are representative of five independent experiments performed in triplicate. (G, H) EP4 mRNA levels in peritoneal macrophages (G) and colonic epithelial cells (H) from WT and KO mice (n = 6 each). Data represent fold induction of mRNA expression compared with WT. All values represent means ± SEM.