Table 2.
Expression of ER and PR in distinct normal mouse mammary epithelial subpopulations
| Markers | Phenotype | Description | ER expression | PR expression | Detection method |
|---|---|---|---|---|---|
| CD24 Sca-1 Prominin-1 [30, 32] |
CD24Low | myoepithelial Cells/myoepithelial Cells | + 0–1 % |
+ | qRT-PCR; IFa (% of positive cells) |
| CD24High Sca-1−/Prominin-1− | Luminal cells (High CFC frequency) | + 1–5 % |
+ | ||
| CD24High Sca-1+/Prominin-1+ | Luminal cells (Low CFC frequency) | +++ 80–90 % |
+++ | ||
| CD45/CD31 (Lin) CD24 CD49f/CD29 [34] |
Lin− CD24+ CD29 Low/CD49f Low | Luminal cells | +++ 37 % |
40 % | qRT-PCR; ICC (% of positive cells) |
| Lin− CD24+ CD29 High/CD49f High | Stem cells | + <0.01 % |
<0.01 % | ||
| CD45/CD31 (Lin) CD24 CD29 CD61 [35, 26] |
Lin− CD24+ CD29 Low CD61− | Luminal cells (Low CFC frequency) | +++ 40 % |
+++ | qRT-PCR; ICC (% of positive cells) |
| Lin− CD24+ CD29 Low CD61+ | Luminal cells (High CFC frequency) | + 6 % |
+ | ||
| CD45 CD49f CD24 Sca-1 c-Kit [31] |
CD45− CD24+/High Sca-1− c-Kit+ | Sca-1− Luminal cells (High CFC frequency) | + 0 % |
NDb | qRT-PCR; IF (% of positive cells) |
| CD45− CD24+/High Sca-1− c-Kit− | Sca-1− Luminal cells (Low CFC frequency) | +++ 100 % |
ND | ||
| CD45− CD24+/High Sca-1+ c-Kit+ | Sca-1+ Luminal cells (High CFC frequency) | +++ 100 % |
ND | ||
| CD45− CD24+/High Sca-1+ c-Kit− | Sca-1+ Luminal cells (Low CFC frequency) | +++ 100 % |
ND | ||
| CD45− CD24+/Low CD49f Low Sca-1−c-Kit− | Myoepithelial cells | + 0 % |
ND | ||
| CD45− CD24+/Low CD49f High Sca-1−c-Kit− | Basal cells (High MRU frequency) | + 0 % |
ND | ||
| CD45− CD24+/Low CD49f Low Sca-1−c-Kit+ | Basal cells (Low MRU frequency) | ++ 0 % |
ND | ||
| CD45/CD31/TER119/ /BP-1 (Lin) EpCAM CD49f CD49b Sca-1 [29] |
Lin− EpCAMHigh CD49fLow Sca-1− CD49b+ | Luminal cells (High CFC frequency) | + 15–25 % |
+ | qRT-PCR; ICC (% of positive cells) |
| Lin− EpCAMHigh CD49fLow Sca-1+ CD49b+ | Luminal cells (Low CFC frequency) | +++ 60–90 % |
+++ | ||
| Lin− EpCAMHigh CD49fLow Sca-1+ CD49b− | Luminal cells (Very low CFC frequency) | +++ 80–90 % |
+++ |
a IF immunofluorescence
b ND not determined