While eliciting pulse trains using ventral root stimulation, loose cell-attached recordings from Renshaw cells showed reliable spiking but with progressive depression as the stimulation frequency increased. The first, second, and 20th responses within a train are illustrated for a Renshaw cell during ventral root stimulation at 10 Hz (A), 33 Hz (C), 50 Hz (E), and 100 Hz (G). B, D, F, H, Respective group data for corresponding frequencies. Group data divided by the different frequencies are illustrated for the second (I, mean ± SEM) and 20th (J, box-and-whiskers) response .