Skip to main content
. 2015 Sep 18;6:8308. doi: 10.1038/ncomms9308

Figure 4. All DnaK variants completely or partially impaired in allosteric regulation had residual chaperone activity in vitro.

Figure 4

(a–c) Refolding of chemically denatured luciferase (80 nM) by DnaK (800 nM), DnaJ (160 nM) and GrpE (400 nM). Luciferase was denatured in 6 M guanidinium HCl and diluted 125-fold into refolding buffer containing the respective DnaK variant, DnaJ and GrpE. Shown is the activity of luciferase relative to the not denatured control. Error bars represent s.e.m. of at least three independent experiments. (d) Aggregation prevention assay. Guanidinium-denatured luciferase (80 nM) was diluted into a solution containing DnaK (800 nM) and DnaJ (40 nM), and light scattering at 600 nm was followed over time.