Table 1. In vivo phenotype of the mutant dnaK alleles.

	IPTG (μM)
	0	50	100	150	250
dnaKwt	—	0.06	1.18	0.67	0.76
Vector	—	—	—	—	—
dnaK-Y145F	—	—	0.0005	0.26	0.68
dnaK-Y145H	—	—	0.97	0.8	0.002
dnaK-Y145A	—	—	—	—	—
dnaK-F146A	—	0.0001	0.045	0.09	0.0002
dnaK-D481A	—	—	—	—	—
dnaK-D481K	—	—	—	—	—
dnaK-V440A	—	0.048	0.69	0.80	0.90
dnaK-L484A	—	0.0005	0.048	0.90	0.95
dnaK-D148A	—	0.0001	0.15	0.67	0.75

IPTG, isopropyl β-D-1-thiogalactopyranoside.

Plating efficiency at 40 °C divided by plating efficiency at 30 °C; —, plating efficiency <10⁻⁴.