Table 2. Stability of the mutant proteins is not severely compromised by the mutational alterations.
| Tm,1 (°C) | Tm,2 (°C) | |
|---|---|---|
| DnaKwt | 41.6±0.2 | 75.5±0.3 |
| DnaK-Y145F | 46.5±0.9 | 77.7±1.0 |
| DnaK-Y145H | 47.2±0.3 | 74.4±0.4 |
| DnaK-Y145A | 47.6±0.1 | 74.5±0.1 |
| DnaK-F146A | 42.0±0.1 | 74.9±0.2 |
| DnaK-D148A | 40.6±0.5 | 72.1±0.3 |
| DnaK-V440A | 45.6±0.3 | 72.7±0.8 |
| DnaK-D481A | 43.3±0.2 | 72.0±0.2 |
| DnaK-D481K | 43.6±0.2 | 72.6±0.4 |
| DnaK-L484A | 47.0±2.0 | 72.7±0.4 |
Tm was determined by fitting the thermal unfolding equation to circular dichroism spectroscopy traces at 222 nm.